… test, based on the induction of increased sensitivity to … of pertussis toxin activity in acellular
pertussis vaccines’), to … a set of vaccines for testing, establish a protocol for spiking these …

… if the use of a standardised protocol could improve the inter-… histamine sensitizing test in
mice and the CHO cell-based assay for the residual toxicity testing of acellular pertussis vaccines…

… Current application of this test is limited to bulk products, … parameter, which might increase
variability in test outcomes. … To improve sensitivity and reduce variability of this assay, we …

… for the histamine sensitisation test (HIST) for acellular pertussis … of new or improved pertussis
vaccines, development and … Protocols for pertussis vaccination vary among countries in …

… pertussis toxin (PTx) is the Histamine Sensitization test (HIST)… Remarkably, the incidence of
pertussis has increased since … and optimization protocols of the vaccine production processes…

… sensitivity of the murine histamine sensitization test (HIST) in detecting minute amounts of
active pertussis toxin (PTx), this animal-based test … increased sensitivity of the mice to histamine…

… use of ACVs the lethal histamine sensitization test (HIST) in mice … assay protocols were not,
however, suitable for testing … was found that the LP test performed better than HIST and CHO …

… for the quality control of pertussis vaccines. Stocks of the first … the induction of increased
sensitivity to histamine by active PT… estimate of the PT activity in a test vaccine [9,10]. Both forms …

… and global replacement of the histamine sensitization test. … form the second generation of
pertussis vaccines to protect … The HIST is based on increased sensitivity to histamine upon …

… worldwide and the histamine sensitisation test (HIST) is … dilution of vaccines would help
to increase test sensitivity. Spiking … to HIST for batch-release of acellular pertussis vaccines. …